Detection and relative quantitation of mRNA for creatine kinase isoenzymes in mRNA from myogenic cell cultures and embryonic chicken tissues.
نویسندگان
چکیده
منابع مشابه
Developmental regulation of creatine kinase isoenzymes in myogenic cell cultures from chicken. Levels of mRNA for creatine kinase subunits M and B.
During the first 2 days of culture of myogenic chicken cells only minute amounts of mRNA for a subunit of creatine kinase, M-CK, could be found in cultures from the 3rd and 4th day; however, a dramatic increase of mRNA for the muscle-specific M-CK was observed. The mRNA for B-CK, another subunit of creatine kinase, was readily detectable in young cultures increased up to the 3rd day in culture ...
متن کاملDevelopmental regulation of creatine kinase isoenzymes in myogenic cell cultures from chicken. Biosynthesis of creatine kinase subunits M and B.
At various intervals after plating, cultures were exposed to 2-h pulses of tritium-labeled leucine. For determination of incorporated radioactivity, the creatine kinases were isolated by specific immunoprecipitation. Sodium dodecyl sulfate-gel electrophoresis was used to separate the two types of creatine kinase subunits MCK and B-CK from each other and their radioactivity was determined in the...
متن کاملTurnover of the creatine kinase subunits in chicken myogenic cell cultures and in fibroblasts.
The rates of degradation of creatine kinase subunits, M-CK and B-CK subunits, were measured in cultured myogenic cells and in subcultured fibroblasts. In differentiated myogenic cells, the myotubes, both M-CK and B-CK subunits are synthesized. Their rates of degradation were compared. The M-CK subunits is slightly more stable and is degraded with an average apparent half-life of 75 h, whereas t...
متن کاملThe primary structure of chicken B-creatine kinase and evidence for heterogeneity of its mRNA.
cDNA clones for chicken B-CK were isolated by immunoscreening from a gizzard cDNA library constructed in the expression vector lambda gtll. The entire coding portion in addition to the complete 3' untranslated region and 42 bp of the 5' noncoding part are represented in the clone H4. On RNA blots H4 insert DNA hybridized to a 1600 bp poly(A)+ RNA from gizzard, brain and heart but not to breast ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1978
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)46964-0